Application

Research CategoryNeuroscience

This Anti-DISC1 Antibody, clone 3D4 is validated for use in IHC (Paraffin), western blotting andICC for the detection of DISC1.

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DISC1 in human cerebellum and human cerebral cortex tissue.
Western Blotting Analysis: A representative lot detected DISC1 in human NLF cell lysate (Leliveld, S. R., et al. (2008). Journal of Neuroscience. 28(15):3839-3845).
Immunocytochemistry Analysis: A representative lot detected DISC1 in transfected cells (Bader, V., et al. (2012). Human Molecular Genetics. 21(20):4406-4418).
Immunohistochemistry Analysis: A representative lot detected DISC1 in human cortical tissue sections (Seshadri, S. et al. (2010). PNAS. 107(12):5622-5627).
Immunohistochemistry Analysis: A representative lot detected DISC1 in human brain tissue (Bader, V., et al. (2012). Human Molecular Genetics. 21(20):4406-4418).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC1 has at least four known isoforms.

Immunogen

Recombinant protein corresponding to human DISC1.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected DISC1 in human cerebral cortex tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

94 kDa calculated